Cygnus hcp kit Cygnus also makes two other CHO HCP kits, Cat# F015 and Cat# F550, our most current, 3rd generation kit. 125ng/mL. pastoris 3rd Generation HCP ELISA Kit provides the specificity and sensitivity (LOD ~0. weight components would be represented. Sep 30, 2024 · This kit was developed using broadly reactive polyclonal antibodies to the hundreds of different host cell proteins (HCPs) and is intended for use in determining the presence of E. Click to visit our website! This CHO HCP 3rd Generation ELISA Kit, Item Number F550-1 will be replacing the current CHO HCP ELISA Kit, 3G, Item Number F550. The Cygnus 3G ELISA and Octet® Anti-CHO HCP Kit results show excellent agreement. coli HCP Control Set , 2G is designed to serve as a control for the F1020 E. 4 ng/ml, LLOQ ~3 ng/ml) to detect HCPs with reproducibility that supports regulatory compliance, aiding in optimal purification process development, process control, routine quality control, and product release testing. Our assay qualification has determined that this kit has an LOD of ~0. Bovine Serum Albumin (BSA) ELISA Kit F030 This kit is intended for use in determining the presence of host cell protein impurities in products manufactured by expression in CHO cells. coli host cell protein contamination in products manufactured by recombinant expression. coli HCP ELISA 2G Kit. Summary and Explanation This kit is intended for use in determining the presence of host cell protein impurities in products manufactured by expression in Vero cells. This kit will be replacing Cygnus Sf9 HCP ELISA Kit, 2G, Item Hybridization of antibody-producing cells with NS/0 cells is one of the most commonly employed systems for the production of humanized and mouse monoclonal antibodies. 910-454-9442 www. MDCK is an acronym for Madin User Protocol: Cygnus Technologies CHO HCP ELISA, 3G vs Competitor Kits Cygnus Technologies CHO HCP ELISA Kit, 3G Competitor B CHO HCP ELISA Kit Competitor CB CHO HCP ELISA Kit Competitor G/R CHO HCP ELISA Protocol Details Assay Preparation All reagents, including ready-to-use CHO HCP Standards and Detection Antibody Which Cygnus CHO HCP kit to use: F1045, CM015, or F550-1? Cygnus currently offers 3 ELISA kits to measure CHO HCPs. Anti-CHO 3G:HRP, 12mL Conjugate Antibody for use in Cygnus CHO 3G HCP ELISA kit F550-1 Anti-CHO:HRP Conjugate, 3G Antibody The store will not work correctly in the case when cookies are disabled. Bio-Techne Corporation and Cygnus Technologies partnered to automate our HEK 293 HCP ELISA, 3G (F650S Resupply) on the ProteinSimple™-branded Ella™ immunoassay platform. The antibodies used in this kit were generated against and affinity purified from HCPs obtained from the spent culture media of a null cell line. Discover our host cell protein (HCP) and DNA impurity detection solutions including ELISA kits, immunoassay reagents, & more. Our original Pichia pastoris HCP ELISA Kit (F140) has broad reactivity to peptide epitopes and a small population of antibodies that recognize Pichia glycosylation epitopes. The store will not work correctly in the case when cookies are disabled. HCP can reduce the efficacy of the therapeutic This kit is for determining the presence of host cell protein impurities in products recombinantly expressed in S. It can also be used to trend the performance of the assay over time. 98. coli HCP E. 7 Product Results This kit has LOD ~0. coli host cell protein contamination in products manufactured by recombinant expression in E. For process purification, ELISA can tell you the total host cell protein to drug substance ratio, and MS supplements HCP analysis this data with the identity and quantity of individual problematic host cell This kit has been specifically developed and validated for use in detecting L. Concentration of 250ng/mL (F143F-1) Mammalian HCP Western Blot Kits. This kit will be replacing Cygnus SP2/0 HCP ELISA Kit, Item # F180. 5 ng/ml. coli HCP 2nd Generation ELISA Kit, Item Number F1020, is intended to replace the current E. coli HCP impurities in products manufactured by recombinant expression. Cygnus ® 1G ELISA Cygnus 2G ELISA Cygnus 3G ELISA Octet Anti-CHO HCP Kit Sample 1 227 803 7712 7530 Sample 2 11 94 282 587 Sample 3 198 290 2491 3628 HCP ELISA Kits Catalog No. This kit is intended for use in determining the presence of host cell protein impurities in products manufactured by expression in Vero cells. Such broadly reactive or "generic" assays are useful for detecting HCPs in process development and quality control. This kit is intended for use in determining the presence of Pichia pastoris protein impurities in products manufactured by recombinant expression in Pichia pastoris host cells. Both Cygnus F650S HEK 293 HCP ELISA Kits, 3G, Original and Resupply 1, have gained global regulatory approvals for multiple commercially available gene and cell therapies. Challenging your assay for each new molecule is essential to selecting the most suitable kit. fluorescens , and S. Summary and Explanation Vero HCPs This kit was developed and validated for use in detecting Staphlococcus aureus HCPs. Cygnus Technologies ECOLI HCP ELISA KIT Supplier: Cygnus Technologies F410 This kit was developed using broadly reactive polyclonal antibodies to the hundreds of different HCPs and is intended for use in determining the presence of E. If your product is an antibody or antigen then we can only provide a Product Specification Sheet. coli HCP 2G Antigen Concentrate Catalog #: F1023X CHO HCP ELISA Kit, 3G | F550-1. Our assay qualification has determined that the LOD for this kit is ~300 pg/ml. Pichia pastoris E. Cell line-specific This simple to use, high throughput, and semi-quantitative assay is a method to aid in optimal purification process development, process control, routine quality control and product lot release. validated HCP ELISA. The Cygnus Technologies HEK 293 HCP Control Set is designed to serve as a control for the F650S HEK 293 HCP ELISA Kit, 3G. Use these highly sensitive ELISA kits to detect and measure host cell proteins and bioprocess impurities. This kit was developed and qualified for detection of HEK HCPs using various in-process and final drug substance samples from real world Cygnus Technologies’ AccuRes™ Host Cell DNA Quantification Kits combine our proprietary DNA extraction procedure with a high-quality probe-based master mix that can detect as little as 0. 1 mL/vial for Cygnus Sf9 HCP ELISA Kit F840 SF9 HCP Standards Set, A-H for F840 kit The store will not work correctly in the case when cookies are disabled. 2 ng/ml and LLOQ ~5ng/ml. Kit F1045, the CHO Lysate HCP ELISA Kit, 2G - replaces the first CHO Lysate kit (F015) developed in 1997. . Cygnus also makes the 3rd generation CHO HCP kit, Cat# F550-1. Concentration of 250ng/mL (F018F-1. Other kits, such as the F550-1 CHO This CHO HCP 3rd Generation ELISA Kit, Item Number F550-1 will be replacing the current CHO HCP ELISA Kit, 3G, Item Number F550. The CAP®cell line, being of human origin, provides recombinant proteins with human-type glycosylation and is commonly employed in the production of recombinant proteins, gene therapy products, and vaccines. To overcome the analytical deficiencies of 2D WB, Cygnus developed Antibody Affinity Extraction with the goals of improving the sensitivity and coverage assessment of the total HCP mixture present in cell culture harvest stream, and to allow for detection and demonstration of reactivity to downstream, process-specific HCPs which are the most important HCPs. The kit is for Research and Manufacturing Use Only processesand is not intended for diagnostic use in humans or animals. Sample Diluent recommended for sample dilutions in the HEK 293 HCP ELISA Kit, F650R, MDCK HCP ELISA Kit, F800 and HeLa HCP ELISA Kit, F810. Date 03DEC2021 This ELISA incorporates some improvements over the earlier kits and is designed to better detect the newer, unnatural constructs of Protein A such as the MabSelect SuRe™ ligand sold by GE Healthcare. Submit. Kit F1045, the CHO Lysate HCP ELISA Kit, 2G ELISA kits for the detection of <1ppm of host cell proteins from P. 3rd Generation Cygnus CHO HCP ELISA Kits by AAE-MS Antibody coverage comparison of polyclonal antibodies supporting the original CHO HCP ELISA Kit, 3G, F550 vs. 4 ng/ml and LLOQ ~3ng/ml. In such cases the absorbance of the undiluted sample may be lower than the highest standard in the kit however these samples will fail to show acceptable dilutional linearity/parallelism as evidenced by an apparent increase in dilution corrected HCP concentration with increasing dilution. Apr 16, 2018 · This procedure is taken from the Cygnus Kit Insert (Attachment 5). Cygnus CHO HCP ELISA, 3G, Item # F550-1, should be used when the protein of interest is secreted into the culture media. standard. pastoris HCP Standard F for Cygnus Technologies F140 Kit. Orthogonal Coverage Analysis by AAE™ Anti-HCP Antibodies are the critical reagents for HCP ELISA. The antibodies have been generated in goats CHO HCP Standard F for Cygnus Technologies F015 Kit. com Summary Discover our host cell protein (HCP) and DNA impurity detection solutions including ELISA kits, immunoassay reagents, & more. coli host cell protein impurities in products manufactured by recombinant expression in BL21(DE3) and Explore our troubleshooting guides, videos, and FAQs for quick solutions, and ways to optimize your next assay. the resupply CHO HCP ELISA Kit, 3G, F550-1 White Paper Written by: Jared Isaac, Alla Zilberman, and Eric Bishop, Cygnus Technologies cygnustechnologies. The CHO HCP Simple Plex Assay, 3G-1 is based on the same antibodies currently used in the Cygnus CHO HCP ELISA Kit, 3G (F550-1). It includes four Anti-CHO Coated Microtiter Strip plates and all reagents and standards in the volumes sufficient to perform CHO HCP ELISA on the Hamilton Microlab® STAR™ Liquid Handling System. HCP in final DS. High Dose Item MRC5 HCP ELISA Kit; Company Cygnus Technologies; Price Supplier Page View Company Product Page; Catalog Number F300; Quantity 96 wells (1 Kit) Applications ELISA; Detection Target MRC5 HCP (Host Cell Protein) Our Host Cell DNA Extraction Kits are compatible with any host cell DNA detection, amplification, and quantification kits. Bishop showed how color coding made the virtual gel images more useful than 2D silver stains, facilitating identification of patterns in missed spots. F233A, with these kits; available separately. We used two different kits to test nine commercial biologics for residual HCP. coli HCPs and performed a comparative coverage analysis study to assess the reactivity of anti-E. This kit is a 2nd Generation method replacing our earlier Catalog # F020, SF9 Insect Cell HCP ELISA kit. The F015 kit should be used when the product is obtained by lysing This kit is intended for use in determining the presence of E. Consult Cygnus Technologies F1055 ® ndPER. AAE is has greatly increased CYGNUS Technologies DNA Kits; CYGNUS Technologies ELISA Kits; CYGNUS Technologies HCP Kits; CYGNUS Technologies Immunogenicity Analysis Kits; CYGNUS Technologies MockV; CYGNUS Technologies Protein A; CYGNUS Technologies Reagents; CYGNUS Technologies Robotics; CYGNUS Technologies Western Blot Kits that particular HCP. The superior co-precipitating reagents do not interfere with qPCR and ensure that pellets do not become dislodged during extraction. 8, 02FEB2018 Certificate of Analysis E. Cygnus has manufactured, characterized, and qualified large pools of the new reagents that will replace the current reagents. 2 ng/ml, LLOQ ~3 ng/ml) to detect HCPs with reproducibility that supports regulatory compliance. The manufacturing and purification process of these products leaves the potential for impurities by host cell proteins (HCPs) from CHO cells. coli HCP ELISA Kit, 2G (Item Number F1020). This is the 3rd Generation ELISA kit for CHO HCP detection. Bacterial HCP ELISA Kits ELISA kits for the detection of <1 ppm host cell proteins from E. As such this kit can be used as a process development tool to monitor the optimal removal of host cell impurities as well as in routine final product release testing. It was qualified for testing of final product HCPs by using actual in-process and final drug substance samples. Each user of this kit is encouraged to perform a similar qualification study to demonstrate it meets their analytical needs. HEK 293 HCP Standards (A-F) HEK 293 HCP Standards set A-F, 6 x 1 ml/Vial: 0, 4, 10, 25, 75 & 200ng/mL for Cygnus HEK293 HCP Kit F650S HEK 293 HCP Standards (A-F) set The store will not work correctly in the case when cookies are disabled. In a recent example, Cygnus Technologies transitioned a depleted Chinese hamster ovary (CHO) HCP antibody and its corresponding CHO HCP ELISA from the original F550 kit to our F550-1 kit. coli HCP Control Antigen Catalog #: F417 The Octet® Anti-CHO HCP Kit has been designed for a rapid high-throughput quantitation of residual CHO Host Cell Proteins (HCPs) in bioprocessing samples during production and development of biopharmaceuticals. Cygnus Technologies, LLC 4332 Southport Supply Rd. coli HCP Standard H Catalog #: F1023H-1 E. For example, add 50 µL of the 200 ng/mL standard to 150 µL of the bufferprovided by the requestor. Offered in three volumes: 100 ml, 500 ml, and 1000 ml. Cygnus Technologies, part of Maravai LifeSciences, offers generic HCP ELISA Kits for 23 different expression plat-forms, advanced orthogonal antibody coverage analysis ser-vices, HCP identification in The Cygnus Technologies E. Bio-Techne Corporation and Cygnus Technologies partnered to automate our CHO HCP ELISA, 3G on the ProteinSimple™-branded Ella™ immunoassay platform. Cygnus Technologies: CHO HCP ELISA 3G (F550) Kit reported higher HCP in two samples. Kits containing the new resupply antibodies maintain the same catalog number, F650S, and are identified by the lot numbers starting with characters “R1”. It should be utilized with the F1020 E. The kit is for Shop Cygnus Technologies F680 HCP ELISA KIT at Fishersci. Our HCP kit is generic in the sense that it is intended to react with essentially all of the HCPs that could contaminate the product, independent of purification process. Our assay validation has determined the LOD for this kit is ~0. The antibodies were generated against and affinity purified using a mild lysate of BHK This kit is intended for use in determining the presence of Human Embryonic Kidney (HEK) host cell protein (HCP) impurities in products manufactured by transgenic expression in HEK host cells. HCP coverage analysis is required for assay qualification as it assesses the ability of the antibodies to recognize a wide range of HCPs in the calibration standard and those present in in-process and drug substance samples. 8 ng/ml. Cytiva: Amersham HCPQuant CHO (Supernatant) Kit reported higher HCP in four samples. Note: HCP immunoassays are complex assays that CHO HCP Standard F Concentration: 100 ng/ml, Cygnus CHO HCP Standard F for F550-1 kit CHO HCP Standard F for F550-1 kit The store will not work correctly in the case when cookies are disabled. This kit has been successfully qualified for a number of different therapeutic human monoclonal antibodies. Demonstrates how to run assays with the Cygnus Technologies' easy-to-use Protein A Mix-N-Go™ ELISA Kits E. 2 Standard Preparation standard in the kit, however, these samples will fail to show acceptable dilution linearity/parallelism as evidenced by an apparent increase in dilution corrected HCP concentration with increasing dilution. Our assay qualification has determined that the LOD for this kit is ~1. The antibodies used in this 2nd generation CHO Lysate HCP ELISA Kit, F1045, have been raised against and affinity purified using a mild lysate of CHO cells. The original kit, Cat# F140, has a very broad reactivity to peptide epitopes and, in addition, This kit is intended for use in determining the presence of E. Shop Cygnus Technologies CHO HCP ELISA Kit, 3rd Generation, More Sensitive than Previous Kits at Fishersci. Summary and Explanation Extraction (AAE). The reagents are generic in the sense that they are intended to react with essentially all the host cell proteins (HCPs) that could contaminate the product independent of the purification process. The array and protocol, the anti-CHO HCP polyclonal antibody from Cygnus Technologies (F550 CHO HCP ELISA, 3G kit) was bound covalently to a chromatography Table 1: Antibody coverage for Chinese hamster ovary (CHO) cells and identification of host-cell proteins (HCPs) from drug-substance samples by the F550 CHO 3G kit SF9 Insect Cell HCP Standards at 0, 1, 3, 6, 12, 25, 50, and 100ng/mL. NC1690152 Cygnus Technologies has partnered to jointly develop the Octet ® Anti-CHO HCP Kit for quantitation of residual HCPs. The A549 cell line (human lung adenocarcinoma epithelial cell line) when applied with viral vectors has shown utility as a production system for various gene therapy agents. HEK 239 HCP ELISA Kit F650R BHK HCP ELISA Kit F510 HeLa HCP ELISA Kit F810 SF9 HCP ELISA Kit F840 Vero HCP ELISA Kit F500 MDCK HCP ELISA Kit F800 A549 HCP ELISA Kit F230 MRC5 HCP ELISA Kit F300 PER. Cygnus Technologies ELISA kits for the detection of host cell proteins from the Sf9 insect cell line. With a flexible deck layout, the Microlab STAR accommodates CHO HCP ELISA, 3G assay protocol. High Dose Hook CHO HCP Standards Set, A-F for Cygnus F015 kit. The MDCK HCP ELISA Kit, provides recombinant proteins with mammalian-type glycosylation and is commonly employed in the production of recombinant proteins, gene therapy products, and vaccines. If the HCP concentration of the undiluted or less Ready-to-use Gyrolab ® & Cygnus solution for detection of HCPs from HEK 293 cells, a common cell line used for vector production of cell and gene therapies, consists of the HEK 293 HCP Assay Reagent Set for Gyrolab ® from Cygnus Technologies and the Gyrolab ® Bioaffy™ 1000 HC Assay Toolbox. aureus HCP ELISA Kit F320 S. Then rely on the experience of Cygnus to help you develop custom, process-specific antibodies later, as needed. This eliminates the need to centrifiuge samples or repeat a run. Offered in three different volumes: 100 ml, 500 ml, 1000 ml. Cygnus Protein A ELISA Kits (F050H, F400, F400Z) and Protein A Mix-N-Go™ ELISA Kits (F600, F610, F740, F910, F950, F965) are designed for robust and sensitive detection of residual native Protein A, natural recombinant Protein A, and other unnatural recombinant Protein A ligands utilized in many commercially available Protein A chromatography resins. This kit is “generic” in the sense that it is intended to react with a majority of the HCPs that could pollute the product independent of the purification process. Summary and Explanation Oct 20, 2022 · To characterize CHO HCP antibodies for F550 and F550-1 kits, Cygnus scientists fractionated samples using the AAE technique, then used an LC-MS analysis to confirm the results. The 3G CHO ELISA rete the product protein such as monoclonal antibodies. The antibodies in the F550 kit were made from HCPs derived from conditioned media. A new pool of anti-CHO HCP antibody was produced. Detect the presence of host cell protein impurities with our range of high-quality HCP ELISA kits specifically designed for your gene expression system. cerevisiae. Remove the required number of microtiter strips from the kit and place them in the provided frame. Insect Cell HCP ELISA Kits - Host Cell Protein ELISA Kits The store will not work correctly in the case when cookies are disabled. 1 Prepare an ELISA Worklist (Attachment 1) by labeling the wells where the samples and standards will be placed. Cygnus sells two ELISA kits for Pichia HCP detection. Users should validate this assay for use with their product samples. Concentrations of 0, 1, 4, 20, 75, 250ng/mL. Which Cygnus CHO HCP kit to use: F1045, CM015, or F550-1? Cygnus currently offers 3 ELISA kits to measure CHO HCPs. The CHO HCP ELISA Kit, 3G (F550-1) can be used as a process development tool to Cygnus provides analytical tools and solutions delivering insights to improve bioprocess development for faster regulatory approval and better clinical outcomes. MS can be used to identify potentially enriched hitchhiker proteins, guide purification process development, and identify HCPs in the final drug substance. DNA Extraction kits are available in two formats: a 96 deep-well format to accommodate high-throughput processing or a tube-based format, giving you flexibility in your experimental workflow. coli Antigen Concentrate w/o BSA Catalog #: F417H E. PG13 cell line is used for cost-effective production of a high-titer retrovirus capable of infecting human cells for gene and cell therapies. This kit is intended for use in determining the presence of Human Embryonic Kidney (HEK) host cell protein (HCP) impurities in products manufactured by transgenic expression in HEK host cells. This is the 3rd Generation ELISA kit The Cygnus Technologies CHO HCP Control Set is designed to serve as a control for the F550-1 CHO HCP ELISA Kit, 3G. The manufacturing and purification process of these products leaves the potential for PG13-derived host cell protein (HCP) impurities. It is derived from the same antibodies and antigen used in the Cygnus 3rd Generation HEK 293 HCP ELISA kit, Cat# F650S. E. C6 2 Generation HCP ELISA Kit Product Insert 3 CYG-LBL-01463 Rev: 00 Eff: 08 Nov 2023 Assay Protocol • The protocol HCP. The manufacturing and purification process of these products leaves the potential for impurities by host cell proteins (HCPs) from C1 cells. coli , L. Sf9 Insect Cell HCP 3rd Generation Immunoenzymetric Assay for the Measurement of Sf9 Insect Cell Host Cell Proteins Catalog # F1040 Intended Use This kit is intended for use in determining the presence of host cell protein contamination in products manufactured by expression in Sf9 insect host cells. The antibodies in the F015 kit Identification of host cell proteins by Antibody Affinity Extraction™ (AAE™) and mass spectrometry (MS) is a highly valuable strategy to ensure that the HCP ELISA is fit for purpose. Cygnus Technologies, LLC. The antibodies in this kit are also affinity purified polyclonals but were made using rather than a gentle cell lysate. Rely on the experience of Cygnus to help you develop custom, process-specific antibodies later, as needed. lactis HCPs as contaminants in products produced by recombinant expression in Lactococcus lactis. ELISA kits for the detection of host cell proteins from the C1 filamentous fungus Thermothelomyces heterothallica. aureus . The Octet platform's rapid high throughput protein analysis combined with the broad HCP recognition and sensitivity of the industry-standard Cygnus 3G anti-CHO HCP antibody bring scientists the best of both worlds for HCP analysis. coli HCP Standard F Catalog #: F413F P. Table 2: In-house bioprocess samples analyzed with different HCP assay kits. aureus HCP ELISA kit Cygnus F320 Mammalian HCP Western Blot Kits. Sep 30, 2024 · This E. Summary and Explanation Cygnus host cell DNA detection kits utilize PicoGreen ® DNA binding dye and include DNA extraction reagents and cell-line specific DNA calibrators to enable quantitative assessments of host cell DNA in your in-process samples. coli HCP ELISA Kit, 2G Catalog #: F1020 E. With ELISAs for most viral vector and vaccine production cell lines, we continue to support your commercialization projects. AAE-MS was used to assess immunoreactivity of both anti-CHO HCP antibodies to CHO HCPs. Click to visit our website! Cygnus Technologies Host Cell Protein (HCP), EndonucleaseGTP ® and other impurity ELISA kits deliver the exceptional reliability you need. CAP® cell line is a CEVEC Pharmaceuticals GmbH core proprietary technology. 3 ng/ml and LLOQ ~3ng/ml. 3 ng/ml, LLOQ ~1 ng/ml) to detect HCPs with reproducibility that supports regulatory compliance. lactis , P. 2 Pipette 100 µL of the Used to measure residual CHO host cell DNA, this kit uses a 2 ml microfuge tube format and includes our proprietary extraction procedure, which does not use iodine or glycogen. The study was carried out using AAE-MS for HCP detection. Cygnus’ proprietary technology has also been utilized to develop over 100 process-specific antibodies and immunoassays for many global biopharmaceutical companies. Cygnus Technologies continues to market two earlier generation kits, Cat #s F015 and CM015 for CHO HCP detection. Pichia pastoris HCP ELISA kit F140 The store will not work correctly in the case when cookies are disabled. While those kits are broadly reactive to CHO particular HCP. This kit is a larger size of the F550-1 kit. Recombinant expression by the C1 filamentous fungus Thermothelomyces heterothallica cell line is a simple and cost-effective method for production of monoclonal antibodies, recombinant proteins, and vaccines. 2 ng/ml. CHO HCP ELISA Kit, 3G is intended for use in determining the presence of host cell protein impurities in products manufactured by expression in the CHO cell line. Yeast HCP Western Blot Kits. com This kit is intended for use in determining the presence of E. To enable our HCP community to seamlessly transition from the F410 kit to the F1020 kit, we quantified and compared the characteristics of the original and new HCP antigen lots of E. com 500-F410, Rev. coli HCP ELISA 2G Kit and its associated protocol. provides the biotechnology and biopharmaceutical industry with products and analytical methods designed 293 HCP, do a 4x dilution using the 200 ng/mL Cygnus, Inc. This new Cygnus Technologies' CHO HCP ELISA Kit, 3G provides the specificity and sensitivity (LOD ~0. BL21(DE3) HCP ELISA Kit Immunoenzymetric Assay for the Measurement of BL21(DE3) Host Cell Proteins, Catalog # F1060 Intended Use This kit is intended for use in determining the presence of BL21(DE3) E. coli HCP ELISA Kit Catalog #: F410 E. cygnustechnologies. Cygnus is an industry pioneer responsible for developing and commercializing the first generic assay kits for HCP detection. This kit has an LOD of ~0. demonstrate that HCP ELISA is fit for purpose, the assay has to be qualified for dilution lin-earity, accuracy, and precision. Cygnus CHO HCP ELISA Kit, 3G provides specificity and sensitivity (LOD ~0. Such under-estimation of total HCP is very minimal in terms of ‘total’ HCP concentrations reported by ELISA. Figure 5 shows the correlation between the results from the two methods, with a correlation coefficient of 0. C6 HCP ELISA Kit Item # F530. Do not vortex infectious samples. Our qualification of the A549 HCP Kit has determined the LOD is ~0. The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. This kit was developed and qualified for detection of HEK HCPs using various in-process and final drug substance samples from real world I700 Sample diluent recommended for sample dilutions in the HEK 293 HCP ELISA Kit, 3G F650S Yes, I would like to receive emails from Cygnus Technologies. Summary and Explanation It is derived from the same antibodies and antigen used in the Cygnus 3rd Generation CHO HCP ELISA Kit, Cat# F550-1 The reagents are "generic” in the sense that they are intended to react with essentially all the host cell proteins (HCPs) that could contaminate the product independent of the purification process. This kit is specifically designed for use to monitor HCP in a process where the CHO cells are lysed as part of the process to recover the product protein of interest. To enable the HCP community to seamlessly transition from the original F550 kit to the F550-1 CHO HCP ELISA 3G, we performed a comparative coverage analysis study to assess reactivity of anti-CHO HCP antibodies that support F550 and F550-1 kits, respectively. This new Cygnus Technologies' BL21(DE3) HCP ELISA Kit provides the specificity and sensitivity (LOD ~0. The suffix of each product name indicates the type of antibody: The BHK (baby hamster kidney fibroblast) cell line is used as an expression system for viral vaccines and other therapeutic proteins. Our kit has been validated for testing of final product HCPs using actual in-process and final drug substance Cygnus’ reputation for quality is recognized by the industry and global regulatory agencies, with several generic HCP ELISA Kits supporting marketed biologics. coli HCP Standards Set, A-F Catalog #: F413 E. If you are using a recombinant form of Protein A with very significant structural differences from natural Protein A such as MabSelect SuRe™ you should use the F400 kit. This produces a 50 ng/mL concentration of HEK293 HCP in the spiked-buffer control sample. com Supplier: Cygnus Technologies F680 This product was recently added by customer request, and is available for your convenience. This kit has LOD ~0. While those kits are broadly reactive to CHO This new Cygnus Technologies' P. SE Southport, NC 28461 USA Tel. It should be utilized with the F550-1 CHO HCP ELISA Kit, 3G and its associated protocol. Mix thoroughly. 6 fg per microliter of host cell DNA. CHO HCP ELISA Kit, 3G | F550-1. coli HCP ELISA Kit, Catalog # F410 Lot # 110121-3 Exp. 5) Recommended sample diluent for the NS/0 HCP ELISA Kit (F220). Regulations require reduction of HCPs to the lowest levels possible. Cygnus: Expression of therapeutic proteins in CHO cells is a cost-effective method for production of commercial quantities of therapeutic mAbs and recombinant proteins. High Dose The kit utilizes industry-standard Cygnus 3G anti-CHO HCP antibody and offers high sensitivity and broad HCP recognition. For ELISA to be quantitative, each individual HCP must have an excess of antibody. The kit utilizes the industry-standard Cygnus 3G Anti-CHO HCP antibody and enables assays with broad HCP recognition, high precision Use these anti-HCP antibodies to produce an assay that is broadly reactive to the greatest number of HCPs possible. Count the microtiter strips needed. This Vero Cell HCP ELISA has an LOD of ~1. CHO HCP ELISA Kit, 3G Catalog #: F550-1 CHO HCP Standard F Catalog #: F553F-1-1 CHO HCP Antigen Concentrate, 3G Catalog #: F553H-1 The kit is for Research and Manufacturing Use Only and is not intended for diagnostic use in humans or animals. If a hook effect is possible, samples should also be assayed diluted. 7. 10% Discount is included on packs of 5 kits. The most significant source of underestimation of HCP by ELISA is not the absence of antibody, but, rather, lack of antibody excess. , resupply It should be utilized with the F650S HEK 293 HCP ELISA Kit, 3G and its associated protocol. coli. Key Features of the Octet ® Anti-CHO antibodies and antigen used in the Cygnus 3rd Generation CHO HCP ELISA kit, Cat# F550-1. Please use Sample Diluent, Item No. Certificates of Analysis (COA) are available for all generic kits and diluent products. com Cygnus Technologies F680 HCP ELISA KIT, Quantity: Each of 1 | Fisher Scientific accessibility menu, dialog, popup Shop Cygnus Technologies CHO HCP ELISA Kit, 3rd Generation, More Sensitive than Previous Kits at Fishersci. I028, with these kits; available separately. 4 ng/ml. coli HCP ELISA Kit, Item Number F410. HEK 293 HCP ELISA Kit, 3G; This kit replaces previous kit F650R; Storage:Standards -10°C to -30°C upon receipt, All other kit reagents 2°C to 8°C; CYGNUS TECHNOLOGIES mfg# F650S Catalog No. Cygnus Sample Diluent Buffer I028 CAP ® Cell Line HCP ELISA Kit Catalog #: F820. Use our generic Host Cell Protein ELISA kits: During process development and process validation to obtain relevant and specific data than can direct process improvement to decrease levels of HCP contamination. The kits feature: 96-well removable strip microplate format - use only as many wells as you need at any one time User Protocol: Cygnus Technologies CHO HCP ELISA, 3G vs Competitor Kits Cygnus Technologies CHO HCP ELISA Kit, 3G Competitor B CHO HCP ELISA Kit Competitor CB CHO HCP ELISA Kit Competitor G/R CHO HCP ELISA Protocol Details Assay Preparation All reagents, including ready-to-use CHO HCP Standards and Detection Antibody Apr 5, 2021 · Cygnus’ reputation for quality is recognized by the industry and global regulatory agencies, with several generic HCP ELISA Kits supporting marketed biologics. pastoris host cell protein contamination in products. Our assay validation has determined that the LOD for this kit is ~0. The PCR amplification kits contain DNA extraction reagents, DNA calibrators, and validated specific primers that are This kit has LOD ~1. Cygnus Technologies S. If you are evaluating Protein A Mix-N-Go™ ELISA Kits. This kit was developed and qualified for detection of HCPs using various inHEK - process and final drug substance samples from real world processes. Our kit has been validated for testing of final product HCPs using actual in-process and final drug substance samples from a drug product. This kit will be replacing Cygnus PER. 6. Mass spectrometry (MS) is an orthogonal method to HCP ELISA that provides in depth data about specific HCPs and improves HCP analysis. Octet ® Anti-CHO HCP Kit includes 1 tray of 96 biosensors, and all calibrators, reagents, and buffers necessary to analyze 96 HCP samples. C6 HCP ELISA Kit F530 CAP HCP ELISA Kit F820 Process Impurity Kits Catalog No. In cases where the recombinant protein product is also glycosylated, there is potential for some cross-reactivity that can result in either an over-estimation of true HCP or indeterminate HCP concentrations, due to the Cygnus Pichia pastoris HCP ELISA Kit F140 determines the presence of p. The Simple Plex HEK 293 HCP 3G Assay is based on the same antibodies currently used in the Cygnus HEK 293 HCP ELISA Kit, 3G (F650S Resupply). It is required to reduce HCP impurities to the lowest levels practical. We strive to provide our customers with a one-stop shop for the entire scientific supplies category. pastoris and S. Summary and Explanation Offered in three different volumes: 100, 500, and 1000 ml. The antibodies in the F015 kit were made using CHO HCPs obtained from a gentle cell lysate. The MRC5 (human fetal lung fibroblast) cell line is a cost-effective method for the production of vaccines and supports the growth of a broad range of viruses. In such cases the absorbance of the undiluted sample may be lower than the highest standard in the kit however, these samples will fail to show acceptable dilutional linearity/parallelism as evidenced by an apparent increase in dilution corrected HCP concentration with increasing dilution. This kit is intended for use in determ ining the presence of Human Embryonic Kidney (HEK) host cell protein (HCP) impurities in products manufactured by transgenic expression in HEK host cells. Gyrolab CHO-HCP E3G Kit and Cygnus CHO HCP ELISA show comparable results for samples collected from different stages of a two-step purification process (see Figure 4). Use an anti-HCP antibody as the key tool for developing broadly reactive or "generic" assays for detecting HCPs in process development and quality control. coli host cell protein impurities in products manufactured by recombinant expression in E. dga qybeyvzt hzw wdjef wxggmcm syan unafr jmyt hshe nyorqoej